Pyrimidine tract binding protein and La autoantigen interact differently with the 5′ untranslated regions of lentiviruses and oncoretrovirus mRNAs

FEBS Letters

Bonnal, Sophie¹  Waysbort, Axel¹  Estève, Jean-Pierre²  Audigier, Sylvie¹  Prats, Anne-Catherine¹ 

[1] INSERM U397, Endocrinologie et Communication Cellulaire, C.H.U. Rangueil, Avenue Jean Poulhès, 31403 Toulouse Cedex 04, France;INSERM U531, Biologie et Pathologie Digestive, Institut Fédératif de Recherche Louis Bugnard IFR31, C.H.U. Rangueil, Avenue Jean Poulhès, 31403 Toulouse Cedex 04, France

关键词: Retrovirus;    Human immunodeficiency virus-1;    Translation initiation;    Internal ribosome entry site;    Pyrimidine tract binding protein;    BIAcore;    Surface plasmon resonance;    RNA–protein interaction;    EMCV;    encephalomyocarditis virus;    HIV;    human immunodeficiency virus;    HTLV;    human T-cell leukemia virus;    IRES;    internal ribosome entry site;    MuLV;    murine leukemia virus;    nt;    nucleotide;    ORF;    open reading frame;    PAGE;    polyacrylamide gel electrophoresis;    PTB;    pyrimidine tract binding protein;    RRL;    rabbit reticulocyte lysate;    SIV;    simian immunodeficiency virus;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(01)02137-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Retrovirus genomic mRNA exhibits a several hundred nucleotides-long untranslated region (5′ UTR) which encloses many control elements required for retrovirus replication. In addition, this 5′ UTR contains translation regulatory elements, such as internal ribosome entry sites (IRESes) that have been described in oncoretroviruses, as well as in lentiviruses. UV cross-linking experiments suggested that the pyrimidine tract binding protein (PTB), a cellular protein known to regulate the activity of several picornaviral IRESes, binds to human T-cell leukemia virus (HTLV)-I RNA but not to lentiviral human immunodeficiency virus (HIV)-1, HIV-2 or simian immunodeficiency virus RNAs. To calculate the affinity of such RNA–protein interactions, we developed a new method based on the BIAcore technology. The absence of affinity of PTB for lentiviral RNAs was confirmed, whereas its affinity for HTLV-I RNAs was 1000-fold lower than for picornaviral RNAs. The BIAcore technology also revealed a significant affinity of the La autoantigen, previously described for its involvement in translational control of viral mRNAs, for HIV-1 and HTLV-I RNAs. Addition of recombinant PTB to in vitro translation experiments weakly enhanced translation initiation in the presence of HTLV-I IRES, suggesting that such an IRES requires additional trans-acting factors.

【 授权许可】

Unknown