Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection

FEBS Letters

Fukuda, Ryo²  Kinoshita, Yoshikazu²  Ishihara, Shunji²  Kawashima, Kousaku²  Kazumori, Hideaki²  Chiba, Tsutomu¹ 

[1] Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan;Second Department of Internal Medicine, Shimane Medical University, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan

关键词: Gastrin receptor;    Proliferating cell;    Neck zone;    Laser capture microdissection;    In situ reverse transcription polymerase chain reaction;    Gastrin;    ECL;    enterochromaffin-like;    LCM;    laser capture microdissection;    HDC;    histidine decarboxylase;    VMAT;    vesicular monoamine transporter;    HB-EGF;    heparin-binding epidermal growth factor-like growth factor;    AR;    amphiregulin;    EGF-R;    epidermal growth factor receptor;   

DOI  :  10.1016/S0014-5793(01)02084-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gastrin stimulates proliferation of progenitor cells in the neck zone of gastric fundic mucosa. However, whether it directly enhances this proliferation through its receptors remains unclear. We investigated the expression of gastrin receptors in neck zone proliferating cells in rat gastric fundic glands using a reverse transcription polymerase chain reaction (RT-PCR) coupled with laser capture microdissection and in situ RT-PCR. Gastrin receptor expression was identified in c-fos-expressing cells located in the neck zone, and results of the RT-PCR analysis argued against contamination by other cells, such as enterochromaffin-like, parietal or D cells. Supporting this finding, gastrin receptor gene expression was identified in the neck zone as well as base glands by in situ RT-PCR. Therefore, it is suggested that proliferating cells in the neck zone are stimulated directly by gastrin via their gastrin receptors.

【 授权许可】

Unknown