Identification of mouse Jun dimerization protein 2 as a novel repressor of ATF‐2

FEBS Letters

Song, Jun³  Ugai, Hideyo³  Nili, Feuerstein⁴  Horikoshi, Masami¹  Jin, Chunyuan³  Yokoyama, Kazunari K.³  Murata, Takehide³  Sun, Kailai² 

[1] Institute of Molecular and Cellular Bioscience, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan;Department of Medical Genetics, China Medical University, Shenyang 110001, PR China;RIKEN, The Institute of Physical and Chemical Research, Tsukuba Insitute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan;Division of Pheumatology, 909 Steller Chance Laboratory, 422 Curie Blv., Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6100, USA

关键词: Activating transcription factor-2;    Transcription factor;    DNA-binding;    cAMP-response element-dependent transcription;   

DOI  :  10.1016/S0014-5793(00)02387-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mouse cDNA that encodes a DNA-binding protein was identified by yeast two-hybrid screening, using activating transcription factor-2 (ATF-2) as the bait. The protein contained a bZIP (basic amino acid-leucine zipper region) domain and its amino acid sequence was almost identical to that of rat Jun dimerization protein 2 (JDP2). Mouse JDP2 interacted with ATF-2 both in vitro and in vivo via its bZIP domain. It was encoded by a single gene and various transcripts were expressed in all tested tissues of adult mice, as well as in embryos, albeit at different levels in various tissues. Furthermore, mouse JDP2 bound to the cAMP-response element (CRE) as a homodimer or as a heterodimer with ATF-2, and repressed CRE-dependent transcription that was mediated by ATF-2. JDP2 was identified as a novel repressor protein that affects ATF-2-mediated transcription.

【 授权许可】

Unknown