Aspirin promotes TFF2 gene activation in human gastric cancer cell lines

FEBS Letters

Al-Azzeh, Ezzal-din¹  Kornberger, Wolfgang¹  Gött, Peter¹  Azarschab, Pia¹ 

[1] Division of Molecular Genetics, Institute of Anthropology and Human Genetics, University of Tübingen, Wilhelmstr. 27, D-72074 Tübingen, Germany

关键词: Trefoil peptide;    Spasmolytic polypeptide;    Aspirin;    Signal transduction;    Transcriptional regulation;    Carcinogenesis;   

DOI  :  10.1016/S0014-5793(00)02422-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trefoil factor family (TFF) peptides promote cell migration, heal the mucosa and may suppress tumor growth. In reporter gene assays we show that aspirin (1–12 mM) evokes a six-fold up-regulation of TFF2, but not TFF1 and TFF3 transcription in human gastrointestinal cell lines. 6 h after application up-regulation of endogenous TFF2 mRNA was observed. TFF2 transcription was enhanced by indomethacin and arachidonic acid but repressed by staurosporine, suggesting mediation via protein kinase C. We mapped an aspirin responding element −546 to −758 bp upstream of TFF2. Up-regulation of TFF2 by aspirin may partially explain the chemopreventive potential of low dose aspirin in gastrointestinal carcinogenesis.

【 授权许可】

Unknown