The membrane‐extrinsic domain of cytochrome b 558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface

FEBS Letters

Baymann, Frauke³  Nitschke, Wolfgang³  Myllykallio, Hannu²  Schütz, Michael³  Schoepp-Cothenet, Barbara³  Brugna, Myriam³  Schmidt, Christian¹ 

[1] Institut für Biochemie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany;Institut de Génétique et Microbiologie, CNRS, URA 2225, Université Paris XI, F-91405 Orsay Cedex, France;Laboratoire de Bioenérgétique et Ingénierie des Protéines, CNRS, UPR 9036, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, F-13402 Marseille Cedex 20, France

关键词: Archaeon;    Cytochrome c y;    Cytochrome b 558/566;    Electron paramagnetic resonance;    Sulfolobus;    cyt;    cytochrome;    EPR;    electron paramagnetic resonance;   

DOI  :  10.1016/S0014-5793(00)02357-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The orientation of the membrane-attached cytochrome b _558/566-haem with respect to the membrane was determined by electron paramagnetic resonance spectroscopy on two-dimensionally ordered oxidised membrane fragments from Sulfolobus acidocaldarius. Unlike the other redox centres in the membrane, the cytochrome b _558/566-haem was found to cover a range of orientations between 25° and 90°. The described results are reminiscent of those obtained on the Rieske cluster of bc complexes and indicate that the membrane-extrinsic domain of cytochrome b _558/566 can perform pivoting motion between two extreme positions. Such a conformational flexibility is likely to play a role in electron transfer with its redox partners.

【 授权许可】

Unknown