On a revised mechanism of side product formation in the lignin peroxidase catalyzed oxidation of veratryl alcohol

FEBS Letters

Franssen, Maurice C.R.¹  ten Have, Rimko² 

[1] Laboratory of Organic Chemistry, Department of Agrotechnology and Nutritional Sciences, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands;Division of Industrial Microbiology, Department of Agrotechnology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands

关键词: Veratryl alcohol;    Lignin;    Manganese;    Lignin peroxidase;    Radical cation;   

DOI  :  10.1016/S0014-5793(00)02379-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The O₂-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H₂O on the VA radical cation (VA ⁺). This initial reaction is unlikely since it would also lead to side product formation in the absence of O₂, which is not the case. In the current mechanism VA ⁺ reacts first with O₂, whereafter H₂O attacks. Furthermore, this paper describes an alternative explanation for the inhibitory effect of Mn²⁺ on VA side product formation. It is proposed that Mn²⁺ reduces reactive intermediates back to VA.

【 授权许可】

Unknown