An intronless gene encoding a poly(A) polymerase is specifically expressed in testis 1

FEBS Letters

Chung, Jae Hoon²  Lee, Young Jae²  Lee, Younghoon¹ 

[1] Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea;Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea

关键词: Intronless gene;    Polyadenylation;    Poly(A) polymerase;    Spermatogenesis;    Testis;    PAP;    poly(A) polymerase;    Papt;    testis-specific poly(A) polymerase;   

DOI  :  10.1016/S0014-5793(00)02367-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous work demonstrated that a single pre-mRNA could generate multiple forms of mammalian poly(A) polymerase mRNAs by alternative splicing or alternative polyadenylation. A cDNA encoding a testis-specific poly(A) polymerase was isolated in this study. The transcription level of Papt in testis of a 2 weeks old mouse was much lower than that of the general poly(A) polymerase gene, Pap. However, the transcription ratio of Papt to Pap was reversed in testis of a 4 weeks old mouse. Transient expression analysis showed that GFP-Papt fusion protein is present both in the nucleus and cytoplasm of HeLa cells. These results suggest that Papt is involved in polyadenylation of transcripts expressed during spermatogenesis.

【 授权许可】

Unknown