Enhanced release of soluble urokinase receptor by endothelial cells in contact with peripheral blood cells

FEBS Letters

Sidenius, Nicolai¹  Vaheri, Antti²  Mustjoki, Satu² 

[1] Department of Molecular Pathology and Medicine, Molecular Genetics Unit, DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy;Haartman Institute, Department of Virology, P.O. Box 21, University of Helsinki, FIN-00014 Helsinki, Finland

关键词: Urokinase receptor;    Soluble urokinase receptor;    Cell–cell contact;    Endothelial cell;    Blood cell;    uPA;    urokinase-type plasminogen activator;    (s)uPAR;    (soluble) uPA receptor;    D1;    domain 1 of uPAR;    D2D3;    domains 2+3 of uPAR;    GPI;    glycosylphosphatidylinositol;    ECM;    extracellular matrix;    PMA;    phorbol 12-myristate 13-acetate;    ELISA;    enzyme-linked immunosorbent assay;   

DOI  :  10.1016/S0014-5793(00)02312-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The urokinase receptor (uPAR) on the cell surface plays an important role in extracellular proteolysis, cell migration and adhesion. Soluble uPAR (suPAR) has been recently discovered in plasma, but its origin is unclear. Our results now demonstrate that both unstimulated blood mononuclear and endothelial cells can release suPAR and that the release is enhanced when either mononuclear cells or thrombocytes are cultured together with endothelial cells. Co-culture without cell–cell contacts fails to enhance suPAR release. We also found suPAR fragments, known to be potent inducers of chemotaxis, in co-culture growth medium samples. Taken together, our results suggest that normal plasma suPAR can be produced by endothelial and mononuclear cells and that suPAR release in cell–cell contacts may have a regulatory role in cell adhesion.

【 授权许可】

Unknown