Immunocontent and secretion of S100B in astrocyte cultures from different brain regions in relation to morphology

FEBS Letters

Mendez, Andreas¹  Pinto, Silvana S.¹  Gonçalves, Carlos A.¹  Wofchuk, Susana¹  Gonçalves, Daniela¹  Rodnight, Richard¹  Karl, Juliana¹  Gottfried, Carmem² 

[1] Departamento de Bioquı́mica, ICBS, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2600-Anexo, 90.035.003 Porto Alegre, Brazil;Departamento de Ciências Fisiológicas, Pontifı́cie Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil

关键词: S100B;    Astrocyte culture;    Brain region;    Glial fibrillary acidic protein;    Morphology;    GFAP;    glial fibrillary acidic protein;    LPA;    lysophosphatidic acid;   

DOI  :  10.1016/S0014-5793(00)02301-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Primary astrocyte cultures prepared from neonatal hippocampus, cerebral cortex and cerebellum were morphologically distinct. Cells from hippocampus and cortex were almost entirely protoplasmic, whereas cerebellar astrocytes had many processes; in the absence of serum these differences were accentuated. We compared the immunocontent and secretion of the mitogenic protein S100B in these cultures. Immunocontent was 2.5 times higher in cerebellar astrocytes than in hippocampal or cortical astrocytes. Cells from all three regions secreted S100B under basal conditions, but the secretion rate was higher in cerebellar astrocytes. Secretion depended on protein synthesis and was increased by incubation with forskolin or lysophosphatidic acid in mechanisms which were additive. The stellate morphology induced by forskolin was reversed by lysophosphatidic acid in hippocampal but not in cerebellar cultures, suggesting that S100B secretion was not associated with a process-bearing phenotype of astrocytes.

【 授权许可】

Unknown