Epigenes: design and construction of new hereditary units

FEBS Letters

Tchuraev, R.N¹  Stupak, I.V¹  Tropynina, T.S¹  Stupak, E.E¹ 

[1] Institute of Biology, Ufa Research Centre, Russian Academy of Sciences, 69 Prospect Oktyabrya, Ufa 450054, Russia

关键词: Genetic network;    Epigene;    Epigenotype;    Recombinant DNA;    Functional state;    Heredity;    IPTG;    isopropyl-β-D-1-thiogalactopyranoside;    MOPS;    3-morpholinopropanesulfonic acid;    MES;    2-morpholinoethanesulfonic acid monohydrate;   

DOI  :  10.1016/S0014-5793(00)02300-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A plasmid digene construction designed before [Tchuraev, R.N. (1982) J. Gen. Biol. 43, 79–87] has been realised, including feedback by repressing proteins with given trigger regime of gene functioning. Experimental tests of the expected epigene properties of the obtained pECPI recombinant plasmid involving lacI and cI₈₅₇ regulatory genes have shown a phenomenon of steady inheritance of two alternative epigenotypes lacI¹cI⁰ and lacI⁰cI¹ , as well as an external toggle switch through metabolitic and temperature signals from one inherited functional state of the cyclic digene system into another. Thus, we have constructed a hereditary unit of a specific kind, namely, a two-component stationary epigene with preset properties.

【 授权许可】

Unknown