Involvement of the p38 mitogen‐activated protein kinase pathway in tissue inhibitor of metalloproteinases‐1‐induced erythroid differentiation

FEBS Letters

Kadri, Zahra¹  Billat, Claudine¹  Mayeux, Patrick²  Boudot, Cédric¹  Haye, Bernard¹  Sowa, Marie-Line¹  Petitfrère, Emmanuelle¹ 

[1] Laboratoire de Biochimie, CNRS FRE-2260, IFR53 Biomolécules, UFR Sciences Exactes et Naturelles, BP 1039, Université de Reims Champagne-Ardenne, F 51687 Reims Cedex 2, France;Institut Cochin de Génétique Moléculaire (ICGM), Institut National de la Santé et de la Recherche Médicale (INSERM U363), Hôpital Cochin, Université René Descartes, 27 rue du Faubourg Saint Jacques, F 75014 Paris, France

关键词: Tissue inhibitor of metalloproteinases-1;    Cell differentiation;    p38 mitogen-activated protein kinase;    TIMP-1;    tissue inhibitor of metalloproteinases-1;    Epo;    erythropoietin;    p38 MAP kinase;    p38 mitogen-activated protein kinase;    ERK;    extracellular signal-regulated kinase;    JNK;    c-Jun N-terminal kinase;   

DOI  :  10.1016/S0014-5793(00)02210-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the role of the mitogen-activated protein (MAP) kinase pathway in tissue inhibitor of metalloproteinases-1 (TIMP-1)-mediated cellular effects in a human erythroleukemic cell line UT-7. We show that TIMP-1 induced both UT-7 cell erythroid differentiation and proliferation and tyrosine phosphorylation of many intracellular proteins. Using a panel of phosphospecific antibodies, we also demonstrate that phosphorylation of the p38 and c-Jun N-terminal kinases is increased by TIMP-1 whereas phosphorylation of extracellular signal-regulated kinase 1/2 is not induced. Moreover, inhibition of the p38 activity by SB203580 significantly reduces erythroid differentiation induced by TIMP-1, suggesting that the p38 MAP kinase pathway is involved in TIMP-1-induced erythroid differentiation.

【 授权许可】

Unknown