Adrenaline potentiates PI 3‐kinase in platelets stimulated with thrombin and SFRLLN: role of secreted ADP

FEBS Letters

Frøyset, Ann Kristin¹  Strand, Ingrid¹  Selheim, Frode¹  Vassbotn, Flemming S¹  Holmsen, Holm¹ 

[1] Department of Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

关键词: Adrenaline;    Phosphoinositide 3-kinase;    ACD;    acid citrate dextrose;    CP;    creatine phosphate;    CPK;    creatine phosphokinase;    GFP;    gel-filtered platelets;    GroPIns;    glycerophosphoinositols;    HPLC;    high performance liquid chromatography;    PI3K;    phosphoinositide 3-kinase;    PtdIns;    phosphatidylinositol;    PtdIns(3;    4)P2;    phosphatidylinositol 3;    4-bisphosphate;    PtdIns(4;    5)P2;    phosphatidylinositol 4;    5-bisphosphate;    PtdIns(3;    4;    5)P3;    phosphatidylinositol 3;    4;    5-trisphosphate;    PRP;    platelet-rich plasma;    TLC;    thin layer chromatography;   

DOI  :  10.1016/S0014-5793(00)02188-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adrenaline significantly potentiated late thrombin- and SFRLLN-induced PtdIns(3,4)P₂ production. Furthermore, the potentiating effect of adrenaline on thrombin-induced PtdIns(3,4)P₂ production was independent on secreted ADP, whereas, the effect of adrenaline on SFRLLN-induced PtdIns(3,4)P₂ production was completely dependent of secreted ADP. However, the ADP-dependent accumulation of PtdIns(3,4)P₂ was not required for irreversible platelet aggregation induced by SFRLLN in the presence of adrenaline. It is concluded that adrenaline can replace secreted ADP to potentiate PtdIns(3,4)P₂ production in thrombin-stimulated but not in SFRLLN-stimulated platelets, thus demonstrating a qualitative difference between platelet stimulation by thrombin and the thrombin receptor activating peptide SFRLLN.

【 授权许可】

Unknown