Caenorhabditis elegans Chk2‐like gene is essential for meiosis but dispensable for DNA repair

FEBS Letters

Aoki, Hidetoshi¹  Mori, Akiyuki¹  Takahashi, Hideyuki¹  Takanami, Takako¹  Sasagawa, Yohei¹  Higashitani, Atsushi¹ 

[1] Institute of Genetic Ecology, Tohoku University, 2-1-1 Katahira, Sendai 980-8577, Japan

关键词: Chiasma;    Pairing;    RNA-mediated interference;    Protein kinase;    Forkhead homology-associated domain;    Chk2/Cds1;   

DOI  :  10.1016/S0014-5793(00)02178-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Chk2-like gene was identified in the genome of Caenorhabditis elegans. The putative gene product, termed Ce-chk-2 consists of 450 amino acid residues, and shows good homology with the Chk2/Cds1 gene family. The results of RNA-mediated interference (RNAi) indicated that the F1 generation from dsRNA injected animals grew to adulthood, but approximately 95% of their eggs (F2) died during early embryogenesis. Among the few surviving progeny, males (XO animals) arose at an abnormally high frequency (30%). In addition, 12 univalents were observed in full grown oocytes of the F1, while six bivalents were normally observed in wild-type oocytes. Ce-chk-2 gene expression increased in the adult stage, and their expression level decreased in the glp-4 mutant, which is defective in germ line proliferation. The radiation sensitivity of F1 embryos carrying Ce-chk-2 RNAi was not significantly affected.

【 授权许可】

Unknown