Carbamate kinase can replace in vivo carbamoyl phosphate synthetase. Implications for the evolution of carbamoyl phosphate biosynthesis

FEBS Letters

Rubio, Vicente¹  Alcántara, Cristina²  Cervera, Javier² 

[1] Instituto de Biomedicina de Valencia (CSIC), C/Jaime Roig 11, 46010 Valencia, Spain;Instituto de Investigaciones Citológicas (FVIB), C/Amadeo de Saboya 4, 46010 Valencia, Spain

关键词: Carbamate kinase;    Carbamoyl phosphate synthesis;    Arginine synthesis;    Pyrimidine synthesis;    Thermophilic archaea;    Pyrococcus furiosus;    CK;    carbamate kinase;    CP;    carbamoyl phosphate;    CPS;    carbamoyl phosphate synthetase;    IPTG;    isopropyl β-D-thiogalactopyranoside;   

DOI  :  10.1016/S0014-5793(00)02168-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The exclusive involvement of carbamate kinase (CK) in fermentative ATP production and of carbamoyl phosphate synthetase (CPS) in the production of carbamoyl phosphate (CP) for pyrimidines and arginine biosynthesis was challenged by the finding of CK as the only activity synthesising CP in the archaea Pyrococcus furiosus and Pyrococcus abyssi. We now show that CK can replace CPS in vivo: transformation of Escherichia coli devoid of the CPS gene with plasmids encoding the CK from P. furiosus or from Enterococcus faecalis (which uses CK for making ATP) restores the ability of CPS-deficient E. coli to grow in the absence of arginine and uracil if ammonia and bicarbonate are present.

【 授权许可】

Unknown