Chicken neuropeptide Y receptor Y2: structural and pharmacological differences to mammalian Y2 1

FEBS Letters

Holmberg, Sara K.S.¹  Salaneck, Erik¹  Boswell, Timothy²  Berglund, Magnus M.¹  Larhammar, Dan¹ 

[1]Department of Neuroscience, Unit of Pharmacology, Uppsala University, P.O. Box 593, SE 751 24 Uppsala, Sweden

[2]Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, UK

关键词: G-protein coupled receptor;    Neuropeptide Y;    Cloning;    Expression;    In situ hybridization;    Chicken;    NPY;    neuropeptide Y;    PYY;    peptide YY;    ch;    chicken;    h;    human;    p;    porcine;    r;    rat;   

DOI  :  10.1016/S0014-5793(00)02164-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report the molecular cloning of the chicken (Gallus gallus) neuropeptide Y (NPY) receptor Y2, the first non-mammalian Y2 receptor. It displays 75–80% identity to mammalian Y2 and has a surprisingly divergent cytoplasmic tail. Expression of the receptor protein in a cell line showed that the receptor did not bind the mammalian Y2 selective antagonist BIIE0246. Furthermore, porcine [Leu³¹, Pro³⁴]NPY, which binds poorly to mammalian Y2, exhibited an unexpectedly high affinity for chicken Y2. In situ hybridisation revealed expression in the hippocampus. Thus, the chicken Y2 receptor exhibits substantial differences with regard to sequence and pharmacological profile in comparison to mammalian Y2 receptors, while the expression pattern in the central nervous system resembles that observed in mammals.

【 授权许可】

Unknown