Two nuclear localization signals are required for nuclear translocation of nuclear factor 1‐A

FEBS Letters

Sakaue, Reiko¹  Tanabe, Atsuhiro¹  Osada, Shigehiro¹  Imagawa, Masayoshi¹  Nishihara, Tsutomu¹ 

[1] Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-Oka, Suita, Osaka 565-0871, Japan

关键词: Nuclear factor 1;    Nuclear localization signal;    Transcription factor;    Translocation;    DNA-binding protein;    NF1;    nuclear factor 1;    NLS;    nuclear localization signal;    EGFP;    enhanced green fluorescent protein;    DAPI;    4;    6-diamino-2-phenylindole;    PCR;    polymerase chain reaction;    GST;    glutathione S-transferase P;    aa;    amino acids;   

DOI  :  10.1016/S0014-5793(00)02119-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1-A, B, C, X). Although DNA-binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1-A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1-A (NF1-A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1-A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.

【 授权许可】

Unknown