FEBS Letters | |
Two nuclear localization signals are required for nuclear translocation of nuclear factor 1‐A | |
Sakaue, Reiko1  Tanabe, Atsuhiro1  Osada, Shigehiro1  Imagawa, Masayoshi1  Nishihara, Tsutomu1  | |
[1] Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-Oka, Suita, Osaka 565-0871, Japan | |
关键词: Nuclear factor 1; Nuclear localization signal; Transcription factor; Translocation; DNA-binding protein; NF1; nuclear factor 1; NLS; nuclear localization signal; EGFP; enhanced green fluorescent protein; DAPI; 4; 6-diamino-2-phenylindole; PCR; polymerase chain reaction; GST; glutathione S-transferase P; aa; amino acids; | |
DOI : 10.1016/S0014-5793(00)02119-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nuclear factor 1 (NF1) proteins are encoded by at least four genes (NF1-A, B, C, X). Although DNA-binding and the transcription regulation domains of these proteins are well characterized, the nuclear localization signals (NLSs) are still unknown in all NF1s. We have identified two NLSs in NF1-A, and both are required for full translocation to the nucleus, although one of them itself has a partial translocation ability. These two NLSs are conserved in all four NF1s. Interestingly, three isoforms of NF1-A (NF1-A1, A2, A4) have two NLSs and translocate completely to the nucleus. In contrast, NF1-A3 lacks the second NLS and partially stays in the cytoplasm. Since NF1s construct homodimer and heterodimer, these findings indicate the differential regulations of the NF1 translocation.
【 授权许可】
Unknown
【 预 览 】
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