Hepatoma cell migration through a mesothelial cell monolayer is inhibited by cyclic AMP‐elevating agents via a Rho‐dependent pathway

FEBS Letters

Iwasaki, Teruo¹  Nakamura, Hiroyuki⁴  Togawa, Atsushi⁴  Mukai, Mutsuko⁴  Tatsuta, Masaharu²  Akedo, Hitoshi⁴  Imamura, Fumio³ 

[1] First Department of Pathology, Hyogo College of Medicine, Nishinomiya 663-8501, Japan;Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan;Department of Pulmonary Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan;Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan

关键词: cAMP;    Rho;    Fibronectin;    Motility;    LPA;   

DOI  :  10.1016/S0014-5793(00)02129-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

1-Oleoyl lysophosphatidic acid (LPA) induces transmonolayer migration (in vitro invasion) of rat ascites hepatoma MM1 cells and their morphological changes leading to the migration. We have previously shown that an LPA analog, palmitoyl cyclic phosphatidic acid (Pal-cPA), suppresses transmonolayer migration of MM1 cells by rapidly increasing the intracellular cyclic AMP (cAMP) concentration. We report here that various cAMP-elevating agents, including dibutyryl cAMP, forskolin, cholera toxin and 3-isobutyl-1-methylxanthine, consistently inhibited LPA-induced transmonolayer migration of MM1 cells. Moreover, pull-down assays for GTP-bound, active RhoA demonstrated that the blockage by cAMP-elevating agents of morphological changes leading to the migration was probably mediated through inhibiting RhoA activation.

【 授权许可】

Unknown