期刊论文详细信息
FEBS Letters | |
Kinetic mechanism of NADH‐enoyl‐ACP reductase from Brassica napus | |
Simon, J.William1  Copse, Catherine L1  Slabas, Antoni R1  Fawcett, Tony1  | |
[1] Department of Biological Sciences, University of Durham, Durham DH1 3LE, UK | |
关键词: Enoyl reductase; Crotonyl-ACP; Crotonyl-coenzyme A; Matrix-assisted laser desorption ionisation mass spectrometry; Brassica napus; ACP; acyl carrier protein; cro-ACP; crotonyl-ACP; cro-CoA; crotonyl-coenzyme A; ENR; enoyl-ACP reductase; enzyme; enoyl-ACP reductase NADH-specific (EC 1.3.1.9); | |
DOI : 10.1016/S0014-5793(00)02128-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Enoyl-ACP reductase, a component of fatty acid synthase, is a target for anti-microbial agents and herbicides. Here we demonstrate the kinetic mechanism to be a compulsory-order ternary complex with NADH binding before the acyl substrate. Matrix-assisted laser desorption ionisation mass spectrometry analysis of enzymatically and synthesised crotonyl-ACP substrate showed the former to contain a single acyl group, whereas the latter contained up to four additional crotonylations. The use of authentic crotonyl-ACP will be important in future kinetic and crystallographic studies.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020309925ZK.pdf | 130KB | download |