Kinetic mechanism of NADH‐enoyl‐ACP reductase from Brassica napus

FEBS Letters

Simon, J.William¹  Copse, Catherine L¹  Slabas, Antoni R¹  Fawcett, Tony¹ 

[1] Department of Biological Sciences, University of Durham, Durham DH1 3LE, UK

关键词: Enoyl reductase;    Crotonyl-ACP;    Crotonyl-coenzyme A;    Matrix-assisted laser desorption ionisation mass spectrometry;    Brassica napus;    ACP;    acyl carrier protein;    cro-ACP;    crotonyl-ACP;    cro-CoA;    crotonyl-coenzyme A;    ENR;    enoyl-ACP reductase;    enzyme;    enoyl-ACP reductase NADH-specific (EC 1.3.1.9);   

DOI  :  10.1016/S0014-5793(00)02128-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Enoyl-ACP reductase, a component of fatty acid synthase, is a target for anti-microbial agents and herbicides. Here we demonstrate the kinetic mechanism to be a compulsory-order ternary complex with NADH binding before the acyl substrate. Matrix-assisted laser desorption ionisation mass spectrometry analysis of enzymatically and synthesised crotonyl-ACP substrate showed the former to contain a single acyl group, whereas the latter contained up to four additional crotonylations. The use of authentic crotonyl-ACP will be important in future kinetic and crystallographic studies.

【 授权许可】

Unknown