Pantetheinase activity of membrane‐bound Vanin‐1: lack of free cysteamine in tissues of Vanin‐1 deficient mice

FEBS Letters

Pitari, , Giuseppina³  Galland, , Franck²  Malergue, , Fabrice²  Maras, Bruno¹  Martin, Florent²  Naquet, Philippe²  Massucci, Maria Teresa³  Chabret, Claude²  Philippe, Jean Marc²  Duprè, Silvestro¹ 

[1] Dipartimento di Scienze Biochimiche ‘A. Rossi Fanelli’ e Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università di Roma ‘La Sapienza’, P. le Aldo Moro 5, 00185 Rome, Italy;Centre d'Immunologie INSERM U136, CNRS UMR 145 de Marseille Luminy, Université de la Méditerranée, Case 906, 13288 Marseille Cedex 9, France;Dipartimento di Biologia di Base ed Applicata Università di L'Aquila, Via Vetoio 67010 Coppito, L'Aquila, Italy

关键词: Vanin;    Pantetheinase;    Ectoenzyme;    Immunoregulation;    Oxidative stress;   

DOI  :  10.1016/S0014-5793(00)02110-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pantetheinase (EC 3.5.1.-) is an ubiquitous enzyme which in vitro has been shown to recycle pantothenic acid (vitamin B5) and to produce cysteamine, a potent anti-oxidant. We show that the Vanin-1 gene encodes pantetheinase widely expressed in mouse tissues: (1) a pantetheinase activity is specifically expressed by Vanin-1 transfectants and is immunodepleted by specific antibodies; (2) Vanin-1 is a GPI-anchored pantetheinase, and consequently an ectoenzyme; (3) Vanin-1 null mice are deficient in membrane-bound pantetheinase activity in kidney and liver; (4) in these organs, a major metabolic consequence is the absence of detectable free cysteamine; this demonstrates that membrane-bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. Since the Vanin-1 molecule was previously shown to be involved in the control of thymus reconstitution following sublethal irradiation in vivo, this raises the possibility that Vanin/pantetheinase might be involved in the regulation of some immune functions maybe in the context of the response to oxidative stress.

【 授权许可】

Unknown