Calcium channel β subunits differentially modulate recovery of the channel from inactivation

FEBS Letters

Greenberg, Robert M¹  Jeziorski, , Michael C¹  Anderson, Peter A.V¹ 

[1] The Whitney Laboratory, University of Florida, 9505 Ocean Shore Blvd., St. Augustine, FL 32086, USA

关键词: Voltage-gated calcium channel;    α-1 subunit;    β subunit;    Inactivation;    L-type current;    R-type current;    cyCav1;    Cyanea capillata L-type calcium channel α1 subunit;    hCav2.3;    human R-type calcium channel (previously called α1E);    cyβ;    Cyanea capillata calcium channel β subunit;    HVA;    high voltage-activated;    LVA;    low voltage-activated;   

DOI  :  10.1016/S0014-5793(00)02098-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the effects of calcium channel β subunits upon the recovery from inactivation of α₁ subunits expressed in Xenopus oocytes. Recovery of the current carried by the L-type α₁ subunit (cyCa_v1) from the jellyfish Cyanea capillata was accelerated by coexpression of any β subunit, but the degree of potentiation differed according to which β isoform was coexpressed. The Cyanea β subunit was most effective, followed by the mammalian b₃, b₄, and β_2a subtypes. Recovery of the human Ca_v2.3 subunit was also modulated by β subunits, but was slowed instead. β₃ was the most potent subunit tested, followed by β₄, then β_2a, which had virtually no effect. These results demonstrate that different β subunit isoforms can affect recovery of the channel to varying degrees, and provide an additional mechanism by which β subunits can differentially regulate α₁ subunits.

【 授权许可】

Unknown