Recombinant C1b domain of PKCδ triggers meiotic maturation upon microinjection in Xenopus laevis oocytes

FEBS Letters

Santos, Eugenio¹  Aroca, Pilar²  Kazanietz, Marcelo G³ 

[1] Centro de Investigación del Cancer, IBMCC, USAL-CSIC, Campus Unamuno, University of Salamanca, E-37007 Salamanca, Spain;Department of Morphological Sciences, Faculty of Medicine, University of Murcia, E-30100 Murcia, Spain;Center for Experimental Therapeutics and Department of Pharmacology, University of Pennsylvania School of Medicine, 816 Biomedical Research Building II/III, 421 Curie Blvd., Philadelphia, PA 19104-6160, USA

关键词: Protein kinase C;    C1 domain;    Oocyte;    Xenopus laevis;    PKC;    protein kinase C;    PDBu;    phorbol 12;    13-dibutyrate;    DAG;    diacylglycerol;    GST;    glutathione S-transferase;    GVBD;    germinal vesicle breakdown;    PI3K;    PI3 kinase;   

DOI  :  10.1016/S0014-5793(00)02075-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The C1 domains are 50 amino acid sequences present in protein kinase C (PKC) isozymes that are responsible for binding of phorbol esters and the lipid second messenger diacylglycerol (DAG). We found that bacterially expressed C1b domain of PKCδ induces germinal vesicle breakdown (GVBD) when microinjected into Xenopus laevis oocytes. Injection of the C1b domain of PKCδ significantly enhanced insulin- but not progesterone-induced maturation. Interestingly, the PKCδ C1b domain markedly synergized with normal Ras protein to induce oocyte maturation when both proteins were co-injected in oocytes. Our results demonstrate that the purified C1b domain of PKCδ is sufficient to promote meiotic maturation of X. laevis oocytes probably through activation of components of the insulin/Ras signaling pathway.

【 授权许可】

Unknown