Mutilation of RNA phage Qβ virus‐like particles: from icosahedrons to rods

FEBS Letters

Petrovskis, Ivars¹  Pumpens, Paul¹  Strelnikova, Anna¹  Ose, Velta¹  Cielens, Indulis¹  Kozlovska, Tatyana¹  Renhofa, Regina¹ 

[1] Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites Street, Riga LV-1067, Latvia

关键词: RNA phage Qβ;    Virus-like particle;    Self-assembly;    Icosahedron;    Rod-like structure;    aa;    amino acid;    CP;    coat protein;    PAGE;    polyacrylamide gel electrophoresis;    VLP;    virus-like particle;    wt;    wild-type;   

DOI  :  10.1016/S0014-5793(00)02061-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Icosahedral virus-like particles (VLPs) of RNA phage Qβ are stabilized by four disulfide bonds of cysteine residues 74 and 80 within the loop between β-strands F and G (FG loop) of the monomeric subunits, which determine the five-fold and quasi-six-fold symmetry contacts of the VLPs. In order to reduce the stability of Qβ VLPs, we mutationally converted the amino acid stretch 76-ANGSCD-81 within the FG loop into the 76-VGGVEL-81 sequence. It led to production in Escherichia coli cells of aberrant rod-like Qβ VLPs, along with normal icosahedral capsids. The length of the rod-like particles exceeded 4–30 times the diameter of icosahedral Qβ VLPs.

【 授权许可】

Unknown