FEBS Letters | |
Mutilation of RNA phage Qβ virus‐like particles: from icosahedrons to rods | |
Petrovskis, Ivars1  Pumpens, Paul1  Strelnikova, Anna1  Ose, Velta1  Cielens, Indulis1  Kozlovska, Tatyana1  Renhofa, Regina1  | |
[1] Biomedical Research and Study Centre, University of Latvia, 1 Ratsupites Street, Riga LV-1067, Latvia | |
关键词: RNA phage Qβ; Virus-like particle; Self-assembly; Icosahedron; Rod-like structure; aa; amino acid; CP; coat protein; PAGE; polyacrylamide gel electrophoresis; VLP; virus-like particle; wt; wild-type; | |
DOI : 10.1016/S0014-5793(00)02061-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Icosahedral virus-like particles (VLPs) of RNA phage Qβ are stabilized by four disulfide bonds of cysteine residues 74 and 80 within the loop between β-strands F and G (FG loop) of the monomeric subunits, which determine the five-fold and quasi-six-fold symmetry contacts of the VLPs. In order to reduce the stability of Qβ VLPs, we mutationally converted the amino acid stretch 76-ANGSCD-81 within the FG loop into the 76-VGGVEL-81 sequence. It led to production in Escherichia coli cells of aberrant rod-like Qβ VLPs, along with normal icosahedral capsids. The length of the rod-like particles exceeded 4–30 times the diameter of icosahedral Qβ VLPs.
【 授权许可】
Unknown
【 预 览 】
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RO201912020309872ZK.pdf | 1030KB | download |