Inhibition of influenza virus replication in cultured cells by RNA‐cleaving DNA enzyme

FEBS Letters

Iwahashi, Jun¹  Ohtsu, Yasushi¹  Toyoda, Tetsuya¹  Takaku, Hiroshi²  Hamada, Nobuyuki¹  Hara, Koyu¹  Tsumura, Naoki³  Kashiwagi, Takahito¹  Imamura, Yoshihiro¹  Kato, Hirohisa³ 

[1] Department of Virology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan;Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Chiba 275-0016, Japan;Department of Pediatrics, Kurume University School of Medicine, Kurume, Fukuoka 830-0011, Japan

关键词: Influenza virus;    RNA-cleaving DNA enzyme;    Antisense oligonucleotide;    PB2;   

DOI  :  10.1016/S0014-5793(00)01974-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Influenza virus replication has been effectively inhibited by antisense phosphothioate oligonucleotides targeting the AUG initiation codon of PB2 mRNA. We designed RNA-cleaving DNA enzymes from 10-23 catalytic motif to target PB2-AUG initiation codon and measured their RNA-cleaving activity in vitro. Although the RNA-cleaving activity was not optimal under physiological conditions, DNA enzymes inhibited viral replication in cultured cells more effectively than antisense phosphothioate oligonucleotides. Our data indicated that DNA enzymes could be useful for the control of viral infection.

【 授权许可】

Unknown