Interaction of human Ku70 with TRF2

FEBS Letters

Song, Kyuyoung³  Lee, Inchul¹  Jung, Donghae¹  Lee, Seong-Gene²  Jung, Yusun² 

[1] Department of Pathology, University of Ulsan College of Medicine, Seoul 138-736, South Korea;Asan Institute for Life Sciences, 388-1 Poongnap-dong, Songpa-Gu, Seoul 138-736, South Korea;Department of Biochemistry, University of Ulsan College of Medicine, Seoul 138-736, South Korea

关键词: Yeast two-hybrid;    Ku;    TRF2;    Telomere;    DNA-PK;    DNA-dependent protein kinase;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulfonyl fluoride;    FITC;    fluorescein isothiocyanate;   

DOI  :  10.1016/S0014-5793(00)01958-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reactive clones were found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70.

【 授权许可】

Unknown