Involvement of an FtsH homologue in the assembly of functional photosystem I in the cyanobacterium Synechocystis sp. PCC 6803

FEBS Letters

Novac, Natalia¹  Robinson, Colin¹  Newman, Julie¹  Bailey, Shaun¹  Mullineaux, Conrad W²  Mann, Nicholas H¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK;Department of Biology, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK

关键词: AAA protease;    Cyanobacterium;    Photosynthesis;    Photosystem I;    Synechocystis;   

DOI  :  10.1016/S0014-5793(00)01871-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Synechocystis sp. PCC 6803 genome encodes four putative homologues of the AAA protease FtsH, two of which (slr0228 and sll1463) have been subjected to insertional mutagenesis in this study. Disruption of sll1463 had no discernible effect but disruption of slr0228 caused a 60% reduction in the abundance of functional photosystem I, without affecting the cellular content of photosystem II or phycobilisomes. Fluorescence and immunoblotting analyses show reductions in PS I polypeptides and possible structural alterations in the residual PS I, indicating an important role for slr0228 in PS I biogenesis.

【 授权许可】

Unknown