Regulation of the biosynthesis of acyl analogs of platelet‐activating factor by purinergic agonist in endothelial cells

FEBS Letters

Balestrieri, Maria Luisa¹  Lee, Ten-ching¹ 

[1] Basic and Applied Research Unit, Oak Ridge Associated Universities, Oak Ridge, TN 37831-0117, USA

关键词: Platelet-activating factor;    Transacetylase;    Acetylhydrolase;    Acyl analog of PAF;    Translocation;    Endothelial cell;    PAF;    platelet-activating factor;    TAL;    PAF: lysophospholipid transacetylase;    TAS;    PAF: sphingosine transacetylase;    AH;    acetylhydrolase;    Acylacetyl-GPC;    1-acyl-2-acetyl-sn-glycero-3-phosphocholine;    BSO;    L-buthionine-(SR)-sulfoximine;    DA;    diamide;    NAC;    N-acetylcysteine;   

DOI  :  10.1016/S0014-5793(00)01870-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that platelet-activating factor (PAF)-dependent transacetylase (TA) contains three catalytic activities, namely PAF: lysophospholipid TA (TA_L), PAF: sphingosine TA (TA_S) and PAF acetylhydrolase. It serves as a modifier of PAF actions by producing different lipid signal molecules. The TA_L activity is involved in the biosynthesis of acyl analogs of PAF (acyl-PAF, 1-acyl-2-acetyl-sn-glycero-3-phosphocholine, acylacetyl-GPC) in agonist-stimulated endothelial cells. In the present investigation, we have studied the mechanism(s) by which the TA activity is regulated in ATP-treated endothelial cells. We have demonstrated that ATP, and thiol-modifying agents with ATP, specifically regulate only the TA_L part of the TA activities.

【 授权许可】

Unknown