A mutation in the pleckstrin homology (PH) domain of the FGD1 gene in an Italian family with faciogenital dysplasia (Aarskog–Scott syndrome)

FEBS Letters

Musacchio, Andrea¹  Bracci, Martina²  Falciani, Michela²  Sorrentino, Vincenzo²  Galli, Lucia²  Orrico, Alfredo²  Cavaliere, Maria Luigia³  Rinaldi, Maria Michela³ 

[1] Department of Experimental Oncology, European Institute of Oncology, Milan, Italy;U.O. Genetica Medica, Policlinico ‘Le Scotte’, Viale Bracci n. 2, 53100 Siena, Italy;Medical Genetics, Azienda Ospedaliera, A. Cardarelli, Napoli, Italy

关键词: Aarskog–Scott disease;    FGD1 gene;    Pleckstrin homology domain;    Missense mutation;   

DOI  :  10.1016/S0014-5793(00)01857-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aarskog–Scott Syndrome (AAS) is an X-linked disorder characterised by short stature and multiple facial, limb and genital abnormalities. A gene, FGD1, altered in a patient with AAS phenotype, has been identified and found to encode a protein with homology to Rho/Rac guanine nucleotide exchange factors (Rho/Rac GEF). However, since this original report on identification of a mutated FGD1 gene in an AAS patient, no additional mutations in the FGD1 gene have been described. We analysed 13 independent patients with clinical diagnosis of AAS. One patient presented a mutation that results in a nucleotide change in exon 10 of the FGD1 gene (G2559>A) substituting a Gln for Arg in position 610. The mutation was found to segregate with the AAS phenotype in affected males and carrier females in the family of this patient. Interestingly, Arg-610 is located within one of the two pleckstrin homology (PH) domains of the FGD1 gene and it corresponds to a highly conserved residue which has been involved in InsP binding in PH domains of other proteins. The same residue is often mutated in the Bruton's tyrosine kinase (Btk) gene in patients with an X-linked agammaglobulinemia. The Arg610Gln mutation represents the first case of a mutation in the PH domain of the FGD1 gene and additional evidence that mutations in PH domains can be associated to human diseases.

【 授权许可】

Unknown