Activated protein C suppresses tissue factor expression on U937 cells in the endothelial protein C receptor‐dependent manner

FEBS Letters

Shu, Fang²  Fukudome, Kenji¹  Terao, Toshihiko²  Kobayashi, Hiroshi²  Kimoto, Masao¹  Tsuneyoshi, Naoko¹ 

[1] Department of Immunology, Saga Medical School, Saga, Japan;Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Japan

关键词: Activated protein C;    Endothelial protein C receptor;    Tissue factor;   

DOI  :  10.1016/S0014-5793(00)01740-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The new functional role of activated protein C (APC) in the regulation of tissue factor (TF) expression was investigated using the cultured human monoblastic leukemia U937 cell line. A flow cytofluorometric analysis demonstrated that treatment with APC resulted in time- and dose-dependent decrease in TF expression in unstimulated and phorbol ester-stimulated cells. The effect was antagonized by the monoclonal antibody (mAb) to endothelial protein C/APC receptor (EPCR), 252, which strongly inhibited the interaction between APC and EPCR. In contrast, mAbs 49 and 379, which bind to EPCR without blocking APC binding, had no or only a modest effect. It is concluded that culturing U937 cells in the presence of APC caused down-regulation of TF expression through the EPCR-dependent mechanism, independent of whether induction was triggered by phorbol ester.

【 授权许可】

Unknown