Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis

FEBS Letters

Arró, Montserrat¹  Ferrer, Albert¹  Forés, Oriol¹  Cunillera, Núria¹  Manzano, David¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain

关键词: cis-Prenyltransferase;    Dehydrodolichyl diphosphate synthase;    Isoprenoid;    Rer2p;    Arabidopsis thaliana;    Dedol-PP;    dehydrodolichyl diphosphate;    ER;    endoplasmic reticulum;    FPP;    farnesyl diphosphate;    IPP;    isopentenyl diphosphate;    PCR;    polymerase chain reaction;    UPS;    undecaprenyl diphosphate synthase;   

DOI  :  10.1016/S0014-5793(00)01798-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The enzyme dehydrodolichyl diphosphate (dedol-PP) synthase is a cis-prenyltransferase that catalyzes the synthesis of dedol-PP, the long-chain polyprenyl diphosphate used as a precursor for the synthesis of dolichyl phosphate. Here we report the cloning and characterization of a cDNA from Arabidopsis thaliana encoding dedol-PP synthase. The identity of the cloned enzyme was confirmed by functional complementation of a yeast mutant strain defective in dedol-PP synthase activity together with the detection of high levels of dedol-PP synthase activity in the transformed yeast mutant. The A. thaliana dedol-PP synthase mRNA was detected at high levels in roots but was hardly detected in flowers, leaves, stems and in A. thaliana suspension-cultured cells.

【 授权许可】

Unknown