Differential immediate‐early gene responses to shear stress and intraluminal pressure in intact human conduit vessels

FEBS Letters

Hägg, Ulrika¹  Johansson, Ann-Marie¹  Doroudi, Roya¹  Jern, Sverker¹  Gan, Li-ming¹  Selin-Sjögren, Lena¹ 

[1] Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Östra, Göteborg University, SE 416 85 Göteborg, Sweden

关键词: Shear stress;    Pressure;    C-jun;    C-fos;    Perfusion;    Real-time PCR;   

DOI  :  10.1016/S0014-5793(00)01788-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown distinct effects of shear stress and pressure on transcription of several potent vascular mediators. In the present study, we tested the hypothesis that c-jun and c-fos are regulated differentially by shear and pressure. Intact human umbilical veins were perfused with various combinations of shear and pressure during 1.5, 3 and 6 h. Protein and gene expressions were assessed by immunofluorescence and real-time reverse transcription PCR, respectively. Shear stress and pressure exert differential temporal effects on c-jun and c-fos gene and protein expression, and these immediate-early gene responses appear to be cell-type specific for endothelial and smooth muscle cells.

【 授权许可】

Unknown