Tissue‐specific expression of multiple mRNA variants of the mouse estrogen receptor α gene

FEBS Letters

Gannon, Frank¹  O'Brien, Sinead¹  Flouriot, Gilles¹  Kos, Martin¹ 

[1] European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany

关键词: Estrogen receptor alpha;    Alternative splicing;    Gene expression;    mERα;    mouse estrogen receptor α;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/S0014-5793(00)01750-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The isolation of five new mouse estrogen receptor α mRNA variants (mERα A, B, F1, F2 and H) is described. All of these transcripts are generated by alternative splicing and all encode the 66 kDa ERα protein that the previously identified mRNA C variant generates. However, these transcripts differ in their 5′ untranslated regions. RT-PCR and S1 nuclease protection assays revealed a tissue- and sex-specific expression pattern of all variants. The C and F mRNA variants are the predominantly expressed mERα variants in mouse. The expression of mERα H mRNA is restricted to liver, although female mice produce around a five fold higher level of this transcript than males. Our results show that the mERα gene is a complex genomic unit in mice that exhibits alternative splicing which is regulated in a tissue-specific manner.

【 授权许可】

Unknown