Decreased expression of the gut‐enriched Krüppel‐like factor gene in intestinal adenomas of multiple intestinal neoplasia mice and in colonic adenomas of familial adenomatous polyposis patients

FEBS Letters

Giardiello, Francis M.¹  Dang, Long H.³  Dang, Duyen T.¹  Yang, Vincent W.¹  Mahatan, Channing S.¹  Bachman, Kurtis E.² 

[1] Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;The Graduate Program in Cellular and Molecular Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA;The Johns Hopkins Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

关键词: Gut-enriched Krüppel-like factor;    Tumorigenesis;    Min mouse;    Familial adenomatous polyposis;    Reverse transcription-polymerase chain reaction;    DNA methylation;    APC;    adenomatous polyposis coli gene;    FAP;    familial adenomatous polyposis;    GKLF;    gut-enriched Krüppel-like factor;    Min;    multiple intestinal neoplasia;    MSP;    methylation-specific PCR;    RT-PCR;    reverse transcription-polymerase chain reaction;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(00)01727-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gut-enriched Krüppel-like factor (GKLF) is a zinc finger-containing transcription factor, the expression of which is associated with growth arrest. We compared Gklf expression in intestinal and colonic adenomas to normal mucosa in multiple intestinal neoplasia (Min) mice and familial adenomatous polyposis (FAP) patients, respectively, using semi-quantitative RT-PCR. In Min mice, the level of Gklf transcript is highest in normal-appearing intestinal tissues and decreases as the size of the adenoma increases. In FAP patients, the level of GKLF transcript is lower in adenomas compared to paired normal-appearing mucosa from the same patient or normal colonic mucosa from control individuals without FAP. The possibility of DNA methylation as a cause for the decreased expression of Gklf in adenomas of Min mice was investigated by methylation-specific PCR. Results indicate that the Gklf gene is not methylated in either normal or tumorous tissues. The findings of our study are therefore consistent with the potential role of GKLF as a negative growth regulator of gut epithelial cells.

【 授权许可】

Unknown