Molecular cloning and functional characterization of the zebrafish ATP‐gated ionotropic receptor P2X3 subunit

FEBS Letters

Cox, Jane A.¹  Egan, Terrance M.¹  Voigt, Mark M.¹ 

[1] Department of Pharmacological and Physiological Sciences, Saint Louis University School of Medicine, 1402 S. Grand Blvd., St. Louis, MO 63104, USA

关键词: Transmitter-gated channel;    Purinoceptor;    Nucleotide;    Zebrafish;   

DOI  :  10.1016/S0014-5793(00)01685-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe a P2X subunit cloned from the zebrafish (Danio rerio) that is an orthologue of the mammalian P2X₃ subunit. Like the mammalian P2X₃, this receptor desensitizes rapidly in the presence of agonist. However, it differs in that αβ-meATP is a much less potent agonist than ATP and the antagonist TNP-ATP is not active at low nanomolar concentrations. Similar to the rat P2X₃ subunit, the zebrafish subunit forms hetero-oligomeric assemblies with the rat P2X₂ that possesses a phenotype distinct from either parent. This novel clone will provide an important basis for future experiments investigating the structure/function relationships of P2X subunit domains.

【 授权许可】

Unknown