A novel Ser O‐glucuronidation in acidic proline‐rich proteins identified by tandem mass spectrometry

FEBS Letters

Bratt, Per¹  Jonsson, Andreas P.²  Bergman, Tomas²  Johansson, Ingegerd¹  J. Griffiths, William²  Strömberg, Nicklas¹  Jörnvall, Hans² 

[1]Department of Cariology, Umeå University, SE-901 87 Umeå, Sweden

[2]Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden

关键词: Tandem mass spectrometry;    Electrospray;    Acidic proline-rich protein;    Post-translational modification;    Phosphorylation;    Glucuronidation;   

DOI  :  10.1016/S0014-5793(00)01645-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human acidic proline-rich salivary protein PRP-1 and its C-terminally truncated form PRP-3 were analyzed by electrospray tandem mass spectrometry. Post-translational modifications were detected and characterized. A pyroglutamic acid residue was demonstrated at the N-terminus, Ser-8 and Ser-22 were shown to be phosphorylated and an O-linked glucuronic acid conjugation was identified. The latter modification was located to Ser-17 and found to be present in approximately 40% of the polypeptides.

【 授权许可】

Unknown