Purification, structure determination and synthesis of covalitoxin‐II, a short insect‐specific neurotoxic peptide from the venom of the Coremiocnemis validus (Singapore tarantula)

FEBS Letters

Bay, Boon-Huat¹  Gopalakrishnakone, P.¹  Sasaki, Toru²  Sato, Kazuki²  Balaji, R.Ashok¹  Kini, R.Manjunatha³ 

[1] Venom and Toxin Research Programme, Department of Anatomy, Faculty of Medicine, National University of Singapore, 4 Medical Drive, Singapore 117597, Singapore;Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo, 194-8511, Japan;Bioscience Centre, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore

关键词: Spider;    Spider venom;    Reversible neurotoxin;    Conus toxin;    Cystine motif;    Cvtx;    covalitoxin;    Fmoc;    9-fluorenylmethoxycarbonyl;    HPLC;    high-pressure liquid chromatography;    ESI/MS;    electrospray ionization mass spectrometry;    MALDI-TOF-MS;    matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;    PTH;    phenylthiohydantoin;    ODS;    octadecylsilane;    TFA;    trifluoroacetic acid;    ACN;    acetonitrile;   

DOI  :  10.1016/S0014-5793(00)01593-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spider venoms contain toxins that specifically immobilize and kill insects. We report the purification and characterization of a new insect-specific toxin named covalitoxin-II (Cvtx-II; mass, 3406.24±0.64), from Coremiocnemis validus (Singapore tarantula) venom. The complete 31 amino acid sequence of Cvtx-II has been determined and it shows less than 40% identity with spider toxins. However, Cvtx-II has conserved cystine motif analogous to other spider and ω-conotoxins. Cvtx-II was chemically synthesized and identified with the native Cvtx-II. Synthetic Cvtx-II induced insect-specific non-lethal excitatory activity when injected into crickets, but not in cockroaches and mice.

【 授权许可】

Unknown