Augmentation of urea‐synthetic capacity by inhibition of nitric oxide synthesis in butyrate‐induced differentiated human hepatocytes

FEBS Letters

Kim, Tae Hun¹  Woo, Gwang Hoon¹  Kim, Chung Yong¹  Lee, Hyo-Suk¹  Yoon, Jung-Hwan¹ 

[1]Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, 28 Yungun-dong Chongno-gu, Seoul 110-744, South Korea

关键词: Nitric oxide;    Urea;    Human;    Hepatocyte;    Butyrate;    Differentiation;    iNOS;    inducible nitric oxide synthase;    SB;    sodium butyrate;    L-NAME;    nitro-L-arginine methyl ester;    AG;    aminoguanidine;    EGF;    epidermal growth factor;    SNP;    sodium nitroprusside;    OTC;    ornithine transcarbamylase;    ASS;    argininosuccinate synthase;    HNF-4;    hepatocyte nuclear factor-4;    C/EBPβ;    CCAAT/enhancer binding protein-β;    NF-κB;    nuclear factor κB;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(00)01599-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate treatment significantly increased the urea-synthetic capacity as compared to that of butyrate treatment alone, possibly through the further induction of ornithine transcarbamylase expression. Therefore, the inhibition of NO production might be useful for obtaining more differentiated hepatocytes in the process of in vitro induction of hepatocyte-specific differentiation.

【 授权许可】

Unknown