A novel fluorescent probe diphenyl‐1‐pyrenylphosphine to follow lipid peroxidation in cell membranes

FEBS Letters

Niki, Etsuo²  Okimoto, Yuko²  Watanabe, Akira²  Yamashita, Takashi¹  Noguchi, Noriko² 

[1]Department of Chemistry and Biotechnology, Faculty of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan

[2]Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan

关键词: Lipid peroxidation;    Polymorphonuclear leukocyte;    Imaging;    Diphenyl-1-pyrenylphosphine;    Fluorescent probe;    tBuOOH;    tert-butyl hydroperoxide;    CCD;    charge-coupled device;    DCFH-DA;    dichlorodihydrofluorescein diacetate;    DHR;    dihydrorhodamine;    DMSO;    dimethyl sulfoxide;    DOPC;    dioleoyl phosphatidylcholine;    DPPP;    diphenyl-1-pyrenylphosphine;    DPPP=O;    diphenyl-1-pyrenylphosphine oxide;    FBS;    fetal bovine serum;    HBSS;    Hanks’ balanced salt solution;    MeLOOH;    methyl linoleate hydroperoxide;    PBS;    phosphate-buffered saline;    PMA;    phorbol 12-myristate 13-acetate;    PMN;    polymorphonuclear leukocyte;   

DOI  :  10.1016/S0014-5793(00)01587-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP=O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.

【 授权许可】

Unknown