14‐3‐3 proteins interact with a 13‐lipoxygenase, but not with a 9‐lipoxygenase

FEBS Letters

Holtman, Wessel L.¹  Testerink, Christa¹  Roberts, Michael R.²  Oppedijk, Berry J.¹  Wang, Mei¹  van Zeijl, Mieke J.¹ 

[1] Center for Phytotechnology UL-TNO, Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands;The Plant Laboratory, Department of Biology, University of York, Heslington, York YO10 5DD, UK

关键词: 14-3-3 protein;    Lipoxygenase;    Interaction;    Lox;    lipoxygenase;   

DOI  :  10.1016/S0014-5793(00)01575-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.

【 授权许可】

Unknown