Inhibition of telomerase activity by a cell‐penetrating peptide nucleic acid construct in human melanoma cells

FEBS Letters

Lualdi, Susanna²  Daidone, Maria Grazia²  Folini, Marco²  Veronese, Silvio¹  Zaffaroni, Nadia²  Villa, Raffaella² 

[1] Department of Pathology, Niguarda Hospital, Milan, Italy;Department of Experimental Oncology, Unit # 10, National Cancer Institute, Via Venezian 1, 20133 Milan, Italy

关键词: Peptide nucleic acid;    Telomerase;    Melanoma cell;    Telomere;    PNA;    peptide nucleic acid;    TRAP;    telomeric repeat amplification protocol;    ITAS;    internal TRAP assay standard;   

DOI  :  10.1016/S0014-5793(00)01540-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening.

【 授权许可】

Unknown