FEBS Letters | |
Inhibition of telomerase activity by a cell‐penetrating peptide nucleic acid construct in human melanoma cells | |
Lualdi, Susanna2  Daidone, Maria Grazia2  Folini, Marco2  Veronese, Silvio1  Zaffaroni, Nadia2  Villa, Raffaella2  | |
[1] Department of Pathology, Niguarda Hospital, Milan, Italy;Department of Experimental Oncology, Unit # 10, National Cancer Institute, Via Venezian 1, 20133 Milan, Italy | |
关键词: Peptide nucleic acid; Telomerase; Melanoma cell; Telomere; PNA; peptide nucleic acid; TRAP; telomeric repeat amplification protocol; ITAS; internal TRAP assay standard; | |
DOI : 10.1016/S0014-5793(00)01540-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020309358ZK.pdf | 495KB | download |