Evidence that 3′‐phosphorylated polyphosphoinositides are generated at the nuclear surface: use of immunostaining technique with monoclonal antibodies specific for PI 3,4‐P2

FEBS Letters

Umeda, Masato⁴  Fukui, Yasuhisa²  Ihara, Sayoko²  Morita, Koji³  Nagata, Satoshi²  Yokogawa, Tohei²  Saitoh, Naoaki¹  Tsutsumi, Tomoaki²  Nishio, Yuichiro²  Shirai, Yasuhito¹  Shirai, Ryuichi³ 

[1] Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe 657-8501, Japan;Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;Department of Molecular Biodynamics, The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan

关键词: Phosphatidylinositol 3;    4-P2;    Monoclonal antibody;    Immunostaining;    Phosphatidylinositol 3-kinase;   

DOI  :  10.1016/S0014-5793(00)01535-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphatidylinositol (PI) 3,4-P₂ is a phosphoinositide that has been shown to be important for signal transduction in growth factor stimulation. We have produced monoclonal antibodies specific for PI 3,4-P₂, which were able to detect PI 3,4-P₂ generated in 293T cells treated with H₂O₂, or in MKN45/BD110 cells expressing activated PI 3-kinase in immunostaining. Prolonged treatment with 0.05% Tween 20 resulted in detection of staining not only at the plasma membrane, but also at the nuclear surface, indicating that 3′-phosphorylated phosphoinositides can be generated and function in the nucleus.

【 授权许可】

Unknown