Genomic structure and promoter analysis of putative mouse acetyl‐CoA transporter gene

FEBS Letters

Hirabayashi, Yoshio¹  Kanamori, Akiko¹  Bora, Roop Singh¹  Ichikawa, Shinichi¹ 

[1]Laboratory for Cellular Glycobiology and Sphingolipid Expression, Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

关键词: Acetyl-CoA transporter;    Transcription factor;    Exon;    Intron;    PCR;    polymerase chain reaction;    Acatn;    acetyl-CoA transporter;    Acatn;    gene encoding Acatn;    DIG;    digoxigenin;    UTR;    untranslated region;    RACE;    rapid amplification of cDNA ends;   

DOI  :  10.1016/S0014-5793(00)01524-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The acetyl-CoA transporter gene (Acatn) encodes a hydrophobic, multitransmembrane protein that is involved in the process of O-acetylation of sialic acid residues on gangliosides. O-Acetylated gangliosides have been found to play important roles in tissue development and organization during early embryonic stages. We have cloned the gene for mouse acetyl-CoA transporter. The gene spans approximately 20 kb and is composed of seven exons and six introns. A single transcription initiation site, 371 bp upstream of the ATG start codon, was identified. The promoter region was found to lack a TATA box. However, several potential transcription factor binding motifs such as AP1, AP2, C/EBPα, C/EBPβ, HSF, GATA2 and MZF1 were identified in the promoter region.

【 授权许可】

Unknown