Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal‐transporting P‐type ATPase ZntA in Escherichia coli

FEBS Letters

Poole, Robert K¹  Binet, Marie R.B¹ 

[1] Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK

关键词: Zn(II);    Cd(II);    Pb(II);    P-type ATPase ZntA;    Transcriptional regulator ZntR;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(00)01509-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

ZntA is a cation-translocating ATPase which exports from Escherichia coli Cd(II) and Pb(II), as well as Zn(II). The metal-dependent ATP hydrolysis activity of purified ZntA was recently characterised and showed a specificity for Cd(II), Pb(II) and Zn(II). zntA expression has been reported to be up-regulated primarily by Zn(II), mediated by the regulatory protein ZntR, belonging to the MerR transcriptional regulator family. In contrast to previous claims, we now show, using a Φ(zntA-lacZ) monolysogen, that Cd(II) is the most effective inducer of zntA, which is also induced significantly by Pb(II). The Cd(II)- and Pb(II)-dependent transcriptional up-regulation of zntA is also mediated by ZntR.

【 授权许可】

Unknown