Accumulation of 210 kDa microtubule‐interacting protein in differentiating P19 embryonal carcinoma cells

FEBS Letters

Dráber, Pavel¹  Zı́ková, Martina¹  Dráberová, Eduarda¹  Sulimenko, Vadym¹ 

[1] Institute of Molecular Genetics, Czech Academy of Sciences, Vı́deňská 1083, 142 20 Praha, 4, Czech Republic

关键词: Antibody;    Microtubule-interacting protein;    P19 cell;    Retinoic acid;    DMSO;    dimethylsulfoxide;    EC;    embryonal carcinoma;    RA;    all-trans-retinoic acid;   

DOI  :  10.1016/S0014-5793(00)01488-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The MA-01 antigen, a thermolabile 210 kDa microtubule-interacting protein, is present in P19 embryonal carcinoma cells on microtubular structures as well as in cytosol. After aggregation of the cells and subsequent incubation with all-trans-retinoic acid (RA), the level of MA-01 expression increased approximately 10 times during 15 days. The increase started after 2 days of incubation with RA and preceded the appearance of neuron-specific tubulin βIII, MAP2C and neurofilaments. Such elevated expression of MA-01 antigen was not detected in P19 cells treated with dimethylsulfoxide. These data indicate that enhanced expression of MA-01 antigen is one of the earliest events occurring in P19 cells during neuronal differentiation.

【 授权许可】

Unknown