Silefrin, a sodefrin‐like pheromone in the abdominal gland of the sword‐tailed newt, Cynops ensicauda

FEBS Letters

Yamamoto, Kazutoshi⁴  Hayashi, Hiroaki⁵  Toyoda, Fumiyo²  Kawai, Yuka⁴  Ohe, Yoshihide¹  Hayashi, Tomoko³  Kawahara, Genri⁴  Kikuyama, Sakaé⁴  Iwata, Takeo⁴ 

[1] Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-0034, Japan;Department of Physiology, Nara Medical University, Kashihara, Nara 634-8521, Japan;Department of Chemistry and Material Science, Gunma College of Technology, Maebashi, Gunma 371-8530, Japan;Department of Biology, School of Education, Waseda University, Nishiwaseda 1-6-1, Shinjuku-ku, Tokyo 169-8050, Japan;Gunma Prefectural College of Science, Maebashi, Gunma 371-0052, Japan

关键词: Sword-tailed newt;    Female-attracting pheromone;    Sodefrin homologue;    Silefrin;    Purification of silefrin;    RIA for silefrin;    Cynops ensicauda;    RIA;    radioimmunoassay;    HPLC;    high-performance liquid chromatography;    cDNA;    complementary DNA;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(00)01455-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sodefrin-like female-attracting pheromone was purified from the abdominal glands of male sword-tailed newts, Cynops ensicauda, by gel-filtration chromatography and reversed-phase high-performance liquid chromatography. The final product comprises 10 amino acid residues with the sequence SILSKDAQLK which coincided with the sequence deduced from its precursor cDNA. This peptide was designated silefrin. The sequence of silefrin was different from that of sodefrin by two amino acid residues, with substitutions Leu for Pro and Gln for Leu at positions 3 and 8, respectively. Both native and synthetic silefrin exerted an equipotent activity in attracting conspecific females.

【 授权许可】

Unknown