SOCS‐1 can suppress CD3ζ‐ and Syk‐mediated NF‐AT activation in a non‐lymphoid cell line

FEBS Letters

Kishi, Hiroyuki¹  Muraguchi, Atsushi¹  Matsuda, Tadashi¹  Yamamoto, Tetsuya¹  Yoshimura, Akihiko² 

[1] Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan;Institute of Life Science, Kurume University, 2432-3 Aikawa-machi, Kurume 839-0861, Japan

关键词: Signal transduction;    T cell antigen receptor;    Syk;    Nuclear factor of activated T cell;    Suppressor of cytokine signaling 1;    Immunoreceptor tyrosine-based activation motif;    PTK;    protein tyrosine kinase;    NF-AT;    nuclear factor of activated T cell;    PLC-γ;    phospholipase C-γ;    ITAM;    immunoreceptor tyrosine-based activation motif;    SOCS;    suppressor of cytokine signaling;    CsA;    cyclosporin A;   

DOI  :  10.1016/S0014-5793(00)01444-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To elucidate T cell antigen receptor (TCR) signaling leading to activation nuclear factor of activated T cells (NF-AT), we reconstituted TCR signaling to activate NF-AT in a non-lymphoid cell line, 293T. We demonstrated that co-expression of CD8/ζ and Syk were necessary for NF-AT activation in 293T. This NF-AT response was completely inhibited by the addition of cyclosporin A or FK506, but markedly enhanced by the additional expression of Tec protein tyrosine kinase. We also show that the cytokine signaling suppressor, suppressor of cytokine signaling 1, potently inhibited this response by interacting with Syk and immunoreceptor tyrosine-based activation motifs in CD8/ζ. These results imply that this novel system may provide a useful tool to delineate or identify the regulatory molecules for CD3ζ/Syk-mediated NF-AT activation.

【 授权许可】

Unknown