Hydrogen exchange monitored by MALDI‐TOF mass spectrometry for rapid characterization of the stability and conformation of proteins

FEBS Letters

Avilés, Francesc X¹  Querol, Enrique¹  Villegas, Virtudes¹  Villanueva, Josep¹  Canals, Francesc¹ 

[1] Institut de Biologia Fonamental and Departament de Bioquı́mica, Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain

关键词: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;    Hydrogen exchange;    Conformational stability;    Potato carboxypeptidase inhibitor;    Activation domain of human procarboxypeptidase A2;    MALDI-TOF MS;    matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;    PCI;    potato carboxypeptidase inhibitor;    ADA2h;    activation domain of human procarboxypeptidase A2;   

DOI  :  10.1016/S0014-5793(00)01418-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used to monitor hydrogen exchange on entire proteins. Two alternative methods have been used to carry out the hydrogen exchange studies, exchanging deuteron (H to D experiments) or proton (D to H experiments). In the former case, the use of a deuterated matrix has made possible to overcome back-exchange problems and attain reproducible results. The methods presented have been used to determine the slow exchange core of the potato carboxypeptidase inhibitor in different folding states, and to differentially compare the activation domain of human procarboxypeptidase A2 versus three site-directed mutants of different conformational stability. In this work, we show that by using MALDI-TOF MS to monitor hydrogen exchange in entire proteins, it is possible to rapidly check the folding state of a protein and characterize mutational effects on protein conformation and stability, while requiring minimal amounts of sample.

【 授权许可】

Unknown