Mammalian calcitonin receptor‐like receptor/receptor activity modifying protein complexes define calcitonin gene‐related peptide and adrenomedullin receptors in Drosophila Schneider 2 cells

FEBS Letters

Born, Walter¹  Gujer, Remo¹  Fischer, Jan A¹  Aldecoa, Amaya¹ 

[1] Research Laboratory for Calcium Metabolism, Departments of Orthopaedic Surgery and Medicine, University of Zurich, Klinik Balgrist, Forchstrasse 340, 8008 Zurich, Switzerland

关键词: Adrenomedullin;    Calcitonin gene-related peptide;    Calcitonin receptor-like receptor;    Receptor activity modifying protein;    Drosophila;    ADM;    adrenomedullin;    CGRP;    calcitonin gene-related peptide;    CRLR;    calcitonin receptor-like receptor;    S2 cells;    Drosophila Schneider 2 cells;    RAMP;    receptor activity modifying protein;   

DOI  :  10.1016/S0014-5793(00)01387-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Differential glycosylation of human and rat (r) calcitonin (CT) receptor-like receptors (CRLR) as a result of interactions with accessory receptor activity-modifying proteins (RAMP)1 or -2 was considered to define CT gene-related peptide (CGRP) or adrenomedullin (ADM) receptors in mammalian cells. Here, Drosophila Schneider (S2) cells stably co-expressed rCRLR and RAMP1 or -2 as functional CGRP or ADM receptors. Different from mammalian cells, rCRLR expressed in S2 cells are uniformly glycosylated proteins independent of RAMP1 or RAMP2. Bis(sulfosuccinimidyl)suberate cross-linking revealed receptor components with the size of rCRLR, increased by the molecular weights of the corresponding RAMPs and [¹²⁵I]CGRP or [¹²⁵I]ADM. In conclusion, [¹²⁵I]CGRP/rCRLR/RAMP1 and [¹²⁵I]ADM/rCRLR/RAMP2 complexes have been recognized in Drosophila S2 cells.

【 授权许可】

Unknown