FHA domain boundaries of the Dun1p and Rad53p cell cycle checkpoint kinases

FEBS Letters

Hammet, Andrew¹  Mitchelhill, Kenneth I.¹  Kemp, Bruce E.¹  Kobe, Bostjan¹  Pike, Brietta L.¹  Heierhorst, Jörg¹  Teh, Trazel¹  House, Colin M.¹ 

[1] St. Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy, Vic. 3065, Australia

关键词: Forkhead-associated domain;    Dun1p;    Rad53p;    Chk2;    Protein kinase;    Cell cycle;    AT;    ataxia–telangiectasia;    FHA;    forkhead-associated;    HU;    hydroxyurea;    KAPP;    kinase-associated protein phosphatase;   

DOI  :  10.1016/S0014-5793(00)01392-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130–140 residues, and are thus considerably larger than previously predicted by sequence comparisons (55–75 residues). In vivo, expression of the proteolytically defined Dun1p FHA domain, but not a fragment containing only the predicted domain boundaries, inhibited the transcriptional induction of repair genes following replication blocks. This indicates that the non-catalytic FHA domain plays an important role in the transcriptional function of the Dun1p protein kinase.

【 授权许可】

Unknown