Characterisation of calmodulin binding to cyclic nucleotide‐gated ion channels from Arabidopsis thaliana

FEBS Letters

Neuhaus, Gunther¹  Köhler, Claudia¹ 

[1] Universität Freiburg, Institut für Biologie II, Zellbiologie, Schänzlestr. 1, D-79104 Freiburg, Germany

关键词: Cyclic nucleotide-gated ion channel;    Calmodulin binding site;    Non-radioactive calmodulin overlay assay;    Calmodulin isoform;    Two-hybrid assay;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(00)01383-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The recently identified cyclic nucleotide-gated ion channels (AtCNGCs) from Arabidopsis thaliana have the ability to bind calmodulin. Using two different methods, we mapped the binding site of AtCNGC1 to the last predicted α helix of the cyclic nucleotide binding domain. This is in contrast to CNGCs from animals, where the calmodulin binding site is located in the N-terminus, implying that different mechanisms for CNGC modulation have evolved in animals and plants. Furthermore, we demonstrate that AtCNGC1 and AtCNGC2 have different calmodulin binding affinities and we provide evidence for target specificities among calmodulin isoforms.

【 授权许可】

Unknown