期刊论文详细信息
FEBS Letters | |
Soluble P‐type ATPase from an archaeon, Methanococcus jannaschii | |
Ogawa, Haruo2  Toyoshima, Chikashi2  Haga, Tatsuya1  | |
[1] Department of Neurochemistry, Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, Japan;Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, Japan | |
关键词: P-type ATPase; Cloning; Overexpression; Ion pump; Methanococcus jannaschii; | |
DOI : 10.1016/S0014-5793(00)01374-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
MJ0968 has been proposed to be an ancestor of P-type ATPase, because its primary structure is highly homologous to that of the core catalytic domain of P-type ATPase. However it completely lacks amino acid sequences that possibly constitute transmembrane domains. To examine if MJ0968 is indeed a P-type ATPase, it was overexpressed in Escherichia coli and purified. It did show ATPase activity, autophosphorylation and inhibition by vanadate. All these properties support the idea that MJ0968 is indeed a soluble P-type ATPase.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020309205ZK.pdf | 180KB | download |